Journal: International Journal of Molecular Sciences

Article Title: Small-Molecule Inhibition of MuRF1 Prevents Early Disuse-Induced Diaphragmatic Dysfunction and Atrophy

doi: 10.3390/ijms24043637

Figure Lengend Snippet: Western blotting analysis of proteins involved in the regulation of skeletal muscle mass and function. ( A ) Representative bands of each protein assessed by Western blot (MW = molecular weight). ( B – D ) Densitometry of total HDAC4, phospho (Ser632) HDAC4, and the ratio of phospho/total HDAC4 protein levels. ( E ) Densitometry of MuRF1 protein levels. ( F ) Densitometry of MuRF2 protein levels. ( G – I ) Densitometry of total FoxO1, phospho (Ser256) FoxO1, and the ratio of phospho/total FoxO1 protein levels. ( J – L ) Densitometry of total Akt, phospho (Ser473) Akt, and the ratio of phospho/total Akt protein levels. Data are shown as mean ± standard error of the mean (* p < 0.05 vs. Sham12h, # p < 0.05 vs. DNV12h + VEH, n = 5–7).

Article Snippet: The following primary antibodies were used: rabbit anti-4-HNE (1:1000, Sigma Aldrich, #393207, St. Louis, MO, USA), rabbit anti-HDAC4 (1:1000, Cell Signaling, #7628, Danvers, MA, USA), rabbit anti-phospho(Ser632) HDAC4 (1:1000; Sigma Aldrich, #SAB4300155), rabbit anti-FoxO1 (1:1000, Cell Signaling, #2880), rabbit anti-phospho(Ser256) FoxO1 (1:000, Cell Signaling, #9461), rabbit anti-Akt (1:000, Cell Signaling, #9272), rabbit anti-phosphor(Ser473) Akt (1:000, Cell Signaling, #4058), rabbit anti-MuRF1 (1:1000, Myomedix, Neckargemünd, Germany), rabbit anti-MuRF2 (1:1000, Myomedix), rabbit anti-GAPDH (1:3000; Cell Signaling, #2118), and rabbit anti-α-tubulin (1:1000, Cell Signaling, #2125).

Techniques: Western Blot, Molecular Weight

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

doi: 10.12659/MSM.910984

Figure Lengend Snippet: TRIM55 is downregulated in HCC tissues and is associated with prognosis of HCC patients. ( A ) IHC detected expression of TRIM55 in HCC tissues and neighboring tissues. Representative photos at 200× and 400×. The level of TRIM55 expression between HCC tissues and neighbor tissues was analyzed and shown as a pie chart. ( B, C ) the relationship between overall survival and TRIM55 expression was analyzed by Kaplan-Meier analysis, and the follow-up data were collected by ourselves ( B ) or TCGA database ( C ).

Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

doi: 10.12659/MSM.910984

Figure Lengend Snippet: Relationship between TRIM55 expression and clinicopathologic features.

Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

doi: 10.12659/MSM.910984

Figure Lengend Snippet: TRIM55 expression is an independent predictive factor for overall survival time in HCC patients.

Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

doi: 10.12659/MSM.910984

Figure Lengend Snippet: TRIM55 overexpression in HCC cell lines was constructed. ( A ) Western blot was used to verify transfection efficiency at the protein level. GAPDH was used as internal control. ( B ) RT-PCR was used to verify transfection efficiency at the mRNA level, *** p<0.0001.

Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

Techniques: Over Expression, Construct, Western Blot, Transfection, Reverse Transcription Polymerase Chain Reaction

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

doi: 10.12659/MSM.910984

Figure Lengend Snippet: Overexpression of TRIM55 inhibits migration and invasion of HCC cells. ( A, B ) Cell migration and invasion ability were detected by Transwell assay in HCC cells with TRIM55 overexpression and negative control. All experiments were repeated 3 times. *** p<0.0001

Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

Techniques: Over Expression, Migration, Transwell Assay, Negative Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

doi: 10.12659/MSM.910984

Figure Lengend Snippet: Overexpression of TRIM55 inhibits migration and invasion of HCC cells through EMT and MMP2. ( A ) IF was used to detect expression of E-cadherin and Vimentin in HCC cells with TRIM55 overexpression and negative control. ( B ) Western blot was used to detect expression of E-cadherin, Vimentin, and MMP2 in HCC cells with TRIM55 overexpression and negative control. GAPDH was used as internal control.

Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

Techniques: Over Expression, Migration, Expressing, Negative Control, Western Blot